Open AccessElectroporation of intact cells of Streptomyces parvulus and Streptomyces vinaceus
Author(s) -
MazyServais Cécile,
Baczkowski Dominique,
Dusart Jean
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12561.x
Subject(s) - streptomyces , electroporation , actinomycetales , streptomycetaceae , microbiology and biotechnology , biology , bacteria , chemistry , biochemistry , genetics , gene
Various assays of classical PEG‐assisted transformation as well as electrotransformation of Streptomyces parvulus IMET41380 and Streptomyces vinaceus NCIB8852 are described. Contrary to the so far reported assays of electrotransforming Streptomyces strains, electroporation in S. parvulus and S. vinaceus was carried out on intact cells, without any lysozyme treatment. In these two strains, the classical PEG‐assisted transformation of protoplasts does not work efficiently (10 3 to 10 4 transformants per μg of pIJ702 DNA) and electrotransformation gives 10 to 100 times higher yields (10 5 transformants per μg of pIJ702 DNA). The electroporation method described here is not applicable to other Streptomyces strains ( S. lividans or S. coelicolor ).