Open AccessBlock of the cell cycle of the yeast Saccharomyces cerevisiae by tyrphostin, an inhibitor of protein tyrosine kinase
Author(s) -
Fujimura Hiroaki
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10487.x
Subject(s) - biology , saccharomyces cerevisiae , tyrosine kinase , receptor tyrosine kinase , biochemistry , tyrosine phosphorylation , phosphorylation , tyrosine , microbiology and biotechnology , yeast , signal transduction
Tyrphostins are inhibitors of the epidermal growth factor receptor tyrosine kinase. To elucidate the biological function of protein tyrosine kinases in yeast cells, a mutant hypersensitive to tyrphostin was isolated and investigated for its response to the drug. The mutation was recessive and was designated tpt1 for t yr p hos t in hypersensitive. A tpt1 strain cannot grow in the presence of tyrphostin, implying that a biological process sensitive to tyrphostin is essential for cell growth. Microscopic observation indicated that large‐budded cells were accumulated in the presence of the inhibitor. The results suggest the involvement of protein tyrosine phosphorylation in the cell cycle progression of Saccharomyces cerevisiae .