Open AccessInduction of stress proteins in the phototrophic bacterium Rhodobacter sphaeroides
Author(s) -
Nepple Bernadette B,
Bachofen Reinhard
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10479.x
Subject(s) - rhodobacter sphaeroides , rhodospirillales , phototroph , bacteria , rhodospirillaceae , gel electrophoresis , phenanthrene , heat shock protein , chemistry , rhodobacter , purple bacteria , biochemistry , photosynthetic reaction centre , biophysics , biology , photosynthesis , mutant , genetics , organic chemistry , gene
The stress response of the phototrophic bacterium Rhodobacter sphaeroides was investigated by two‐dimensional gel electrophoresis. Oxygen, 0.5% and 4% ethanol, UV radiation, heat shock at 42°C and 0.01% phenanthrene were tested as stress factors. The protein pattern on two‐dimensional gels of stressed as compared to control cells revealed that all stress factors applied caused modifications in the protein pattern of R. sphaeroides . The intensity of particular spots increased or decreased as a consequence of altered protein synthesis. Specific and general stress responses were observed.