Open AccessNeomycin‐ and spectinomycin‐resistance replacement vectors for Bacillus subtilis
Author(s) -
Chary Vasant K,
Amaya Edward I,
Piggot Patrick J
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10474.x
Subject(s) - bacillus subtilis , plasmid , spectinomycin , neomycin , biology , gene , microbiology and biotechnology , mutagenesis , genetics , antibiotic resistance , bacteria , antibiotics , mutation
A plasmid is described for Bacillus subtilis that facilitates replacement of the widely used neomycin resistance gene ( neo ) with a spectinomycin resistance ( spc E ) gene. A second plasmid is described that facilitates replacement of spc S , associated with mini‐Tn 10 mutagenesis in B. subtilis , with neo . These plasmids can also function as integrative vectors for B. subtilis . They expand the scope of strain construction and gene analysis in B. subtilis .