Open AccessIdentification and characterization of the gyrB gene from Treponema pallidum subsp. pallidum
Author(s) -
Stamm Lola V,
Greene Shermalyn R,
Barnes Natalie Y
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10473.x
Subject(s) - dna gyrase , treponema , gene , biology , homology (biology) , microbiology and biotechnology , nucleic acid sequence , genetics , peptide sequence , dna , escherichia coli , virology , syphilis , human immunodeficiency virus (hiv)
Abstract The nucleotide sequence of a DNA gyrase B subunit gene ( gyrB ) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The organization of genes flanking the gyrB gene is unique in comparison to that of other bacteria. The gyrB gene encodes a 637 amino acid protein whose deduced sequence has a high degree of homology with type‐II topoisomerase ATPase subunits (GyrB and ParE). Five type‐II topoisomerase motifs, an ATP‐binding site (Walker A), and amino acid residues that putatively interact with ATP, are highly conserved in the T. pallidum GyrB protein.