Open AccessA helicase gene ( helO ) in Rhizobium meliloti WSM419
Author(s) -
Reeve Wayne G,
Tiwari Ravi P,
Dilworth Michael J,
Glenn Andrew R
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10461.x
Subject(s) - helicase , gene , rhizobium , biology , dna , amino acid , gene product , chemistry , genetics , biochemistry , gene expression , rna
A 2.8 kb Bam HI DNA fragment adjacent to a Bam HI fragment containing actR ‐ actS (a sensor/regulator pair required for low pH tolerance in Rhizobium meliloti WSM419) was cloned and sequenced. A computer predicted protein of 821 amino acids, designated HelO, showed extensive similarity with ‘DEAH’ motif helicases. Expression of helO was higher at pH 7.0 than pH 5.8 and it did not require the product of the actR gene. Inactivation of helO by insertion of a Ω interposon at codon 40 did not affect nodulation, growth or tolerance to low pH, high temperature, osmolarity or elevated levels of copper or zinc.