Open AccessFluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae
Author(s) -
Aon Juan Carlos,
Cortassa Sonia
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10458.x
Subject(s) - saccharomyces cerevisiae , spore , intracellular , extracellular , intracellular ph , fluorescence , biochemistry , chemistry , biophysics , biology , chromatography , microbiology and biotechnology , yeast , physics , quantum mechanics
This work reports the intracellular pH (pH i ) dynamics of Saccharomyces cerevisiae cells in sporulation medium. Cells loaded with the pH‐sensitive dye carboxy‐seminaphthorhodafluor‐1 (C.SNARF‐1) exhibited an alkalization of the pH i following the extracellular pH during sporulation in the absence of buffer and almost no change in pH i or ΔpH when sporulation was carried out in buffered medium. The results indicate that the pH gradient does not appear to be directly involved in the regulation of acetate uptake during sporulation. However, the alkalization of pH i by eliciting a decrease in metabolic fluxes could account for a lower demand for acetate.