Open AccessPolycaprolactone depolymerase produced by the bacterium Alcaligenes faecalis
Author(s) -
Oda Yuji,
Oida Naoya,
Urakami Teizi,
Tonomura Kenzo
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10449.x
Subject(s) - alcaligenes , alcaligenes faecalis , bacteria , microbiology and biotechnology , chemistry , biology , genetics , pseudomonas
Several microorganisms were isolated as bacteria degrading polycaprolactone (PCL), and one of them, a strain B273 identified as Alcaligenes faecalis , was selected. Because this strain produced only slight PCL depolymerase activity, the hyper‐producing mutant, TS22, was isolated after UV irradiation. Synthesis of PCL depolymerase was derepressed, probably based on the altered regulation of metabolic pathways in strain TS22. The partially purified enzyme hydrolyzed p ‐nitrophenyl fatty acids and triglycerides other than PCL, but not poly(3‐hydroxybutyrate), indicating that PCL depolymerase may be a kind of lipase.