Open AccessExpression of P, S, and F1C adhesins by cytotoxic necrotizing factor 1‐producing Escherichia coli from septicemic and diarrheic pigs
Author(s) -
Dozois Charles M,
Clément Sébastien,
Desautels Clarisse,
Oswald Eric,
Fairbrother John M
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10444.x
Subject(s) - bacterial adhesin , fimbria , microbiology and biotechnology , biology , immunofluorescence , agglutination (biology) , hemagglutination , escherichia coli , antiserum , serotype , virology , antibody , gene , immunology , virus , biochemistry
Nineteen papC ‐positive cytotoxic necrotizing factor 1 (CNF1)‐producing Escherichia coli isolates from pigs with septicemia or diarrhea were tested for the presence of pap ‐, sfa ‐, and afa ‐related sequences encoding P/Prs, S/F1C, and Dr/AFA adhesins respectively. Production of adhesins by isolates was tested by mannose‐resistant hemagglutination (MRHA), sialidase treatment of erythrocytes and particle agglutination tests. Production of P, S, and F1C fimbriae by isolates was also examined by immunofluorescence. All isolates were pap + by PCR. Eighteen isolates (95%) were MRHA for ovine and human A erythrocytes and exhibited GalNac‐GalNac receptor specificity associated with class III P(Prs) adhesins. Fifteen (79%) of the 19 isolates reacted with antisera specific for one or more different P fimbrial serotypes on immunofluorescence. Three of these isolates also demonstrated Gal‐Gal receptor specificity associated with class I or II P fimbrial adhesins. Fifteen (79%) of the isolates were sfa + by PCR. Seven of these isolates exhibited sialidase‐sensitive MRHA of bovine and human O erythrocytes and reacted with serum specific for S fimbriae on immunofluorescence. Seven of the 8 sfa + isolates which were MRHA‐negative for bovine erythrocytes reacted with serum specific for F1C fimbriae on immunofluorescence. All isolates produced type 1 fimbriae as determined by mannose‐sensitive agglutination of yeast cells. None of the isolates were afa + by PCR or colony hybridization. Results suggest that most pap + porcine CNF1‐producing E. coli isolates express P fimbriae bearing class III (Prs) type adhesins. In addition, most of these isolates also produce S or F1C fimbriae.