Open AccessLocalization of germination‐specific spore‐lytic enzymes in Clostridium perfringens S40 spores detected by immunoelectron microscopy
Author(s) -
Miyata Shigeru,
Kozuka Satoshi,
Yasuda Yoko,
Chen Yinghua,
Moriyama Ryuichi,
Tochikubo Kunio,
Makino Shio
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10434.x
Subject(s) - immunoelectron microscopy , spore , immunogold labelling , microbiology and biotechnology , spore germination , germination , lysozyme , clostridium perfringens , biology , antiserum , lytic cycle , biochemistry , bacteria , virology , botany , ultrastructure , antigen , antibody , virus , immunology , genetics
The localization of germination‐specific spore‐lytic enzymes, an amidase and a muramidase, in Clostridium perfringens S40 spores was examined by immunoelectron microscopy with respective antisera raised against the enzymes and a colloidal gold‐immunoglobulin G complex. For both antisera, immunogold particles were visualized on the outside of the cortex of dormant spores, and they were not detected in germinated spores and decoated spores.