Open AccessAmended structure of side chains in a cell wall mannan from Candida albicans serotype A strain grown in yeast extract‐Sabouraud liquid medium under acidic conditions: detection of the branched side chains corresponding to antigenic factor 4
Author(s) -
Kobayashi Hidemitsu,
Tanaka Shizu,
Suzuki Junko,
Kiuchi Yoko,
Shibata Nobuyuki,
Suzuki Shigeo,
Okawa Yoshio
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10433.x
Subject(s) - mannan , side chain , mannose , yeast , candida albicans , antiserum , chemistry , cell wall , stereochemistry , polysaccharide , biochemistry , microbiology and biotechnology , biology , organic chemistry , antibody , polymer , immunology
In a previous study, we reported the excess production of α‐1,3‐linked mannose residues with the complete disappearance of β‐1,2‐linked mannose residues in cell wall mannans of Candida albicans serotype A strain cells, which were grown in yeast extract‐Sabouraud liquid medium at pH 2.0. In the present study, we examined the immunochemical reactivity of the same mannan of NIH A‐207 with an enzyme‐linked immunosorbent assay (ELISA) using several antisera to antigenic factors of the genus Candida (FAbs) and the structure of the mannan by two‐dimensional homonuclear Hartmann‐Hahn analysis. The ELISA showed that the mannan reacts to FAb 4 but not to FAbs 13b and 34, which are reported to be antibody factors against linear side chains containing an α‐1,3‐linked mannose residue. In the Hartmann‐Hahn analysis, we found two branched side chains, Manα1–2Manα1–3[Manα1–6]Manα1‐(2Manα1‐) 2 2Man and Manα1–3[Manα1–6]Manα1‐(2Manα1‐) 2 2Man, instead of the previously reported linear side chains. The branched side chains are oversynthesized under acidic conditions.