Open AccessCloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU‐C12
Author(s) -
Arai Tsuyoshi,
Yusa Satoshi,
Kirimura Kohtaro,
Usami Shoji
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10426.x
Subject(s) - complementary dna , cdna library , microbiology and biotechnology , trichosporon , biology , lipase , peptide sequence , amino acid , molecular cloning , signal peptide , biochemistry , nucleic acid sequence , geotrichum , rapid amplification of cdna ends , enzyme , gene , yeast
A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU‐C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt10‐based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5% overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU‐C12 contained two different lipase genes.