Open AccessCharacterisation of the peptidoglycan hydrolases of Listeria monocytogenes EGD
Author(s) -
McLaughlan Anna M,
Foster Simon J
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10421.x
Subject(s) - peptidoglycan , listeria monocytogenes , microbiology and biotechnology , gel electrophoresis , sodium dodecyl sulfate , enzyme , cell envelope , biochemistry , listeria , chemistry , biology , bacteria , hydrolase , escherichia coli , gene , genetics
Abstract The peptidoglycan hydrolase profile of Listeria monocytogenes EGD has been characterised under a variety of environmental and physiological conditions, using renaturing sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The profiles show activities ranging from 29 to 186 kDa. The 186‐kDa enzyme was only observable under specific medium and aeration conditions. The enzyme activities show differential substrate specificity and sensitivity to incubation conditions. The peptidoglycan hydrolase profile of several different Listeria strains was also compared.