Open AccessT‐track PCR fingerprinting for the rapid detection of genetic polymorphism
Author(s) -
Seong SeungYong,
Park SaeGwang,
Huh MyungSuk,
Choi MyungSik,
Chang WooHyun,
Kim IkSang
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10406.x
Subject(s) - genotyping , biology , genetics , polymerase chain reaction , restriction enzyme , dna profiling , restriction fragment length polymorphism , microbiology and biotechnology , dna , genotype , gene
The diversity of DNA sequences can be analyzed by comparing randomly amplified polymorphic DNA, or restriction fragment length polymorphism fragments of DNA. Such analyses are dependent on the selection of appropriate restriction enzyme(s) and/or primers. We have investigated a simpler approach to providing sensitive and specific genotyping. Cyclic extension of target sequences with dideoxythymidine generates PCR products with variable lengths. We analyzed these variable PCR products by scoring the number of variable bands and comparing the scores (numerical profiles) to establish similarities. We found that the polymorphic lengths of the PCR products were comparable among serologically defined strains. It suggests that this single PCR reaction followed by a one‐step electrophoresis yields easily analyzable data that can be compared with data from other gels.