Open AccessInteraction between Actinobacillus actinomycetemcomitans lipopolysaccharides and human hemoglobin
Author(s) -
Grenier Daniel,
Leduc Annie,
Mayrand Denis
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10397.x
Subject(s) - actinobacillus , hemoglobin , bacteria , microbiology and biotechnology , chemistry , sodium dodecyl sulfate , gel electrophoresis , polyacrylamide gel electrophoresis , pasteurellaceae , biochemistry , biology , antibiotics , enzyme , genetics , haemophilus influenzae
Actinobacillus actinomycetemcomitans , a bacterium associated with juvenile periodontitis, was found to use human hemoglobin as a source of iron for cell growth. Cultivation in the presence of hemoglobin had only a slight effect on the cellular protein pattern, as determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis. Lipopolysaccharides obtained from A. actinomycetemcomitans were found to have a strong capacity to bind hemoglobin. This interaction appears not to involve the lipid A portion of the molecule as no inhibition was obtained when lipopolysaccharides were pre‐treated with polymyxin B. This interaction between hemoglobin and lipopolysaccharides of A. actinomycetemcomitans may facilitate iron acquisition by this bacterium.