Open AccessStructural characterization of lipid A obtained from Pantoea agglomerans lipopolysaccharide
Author(s) -
Tsukioka Daisuke,
Nishizawa Takashi,
Miyase Toshio,
Achiwa Kazuo,
Suda Takuya,
Soma GenIchiro,
Mizuno Den'ichi
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10335.x
Subject(s) - pantoea agglomerans , pantoea , lipopolysaccharide , lipid a , microbiology and biotechnology , characterization (materials science) , chemistry , bacteria , biology , nanotechnology , pseudomonas , materials science , immunology , genetics
Lipopolysaccharide isolated from Pantoea agglomerans showed higher priming and triggering activities for macrophages in terms of tumor necrosis factor production than other lipopolysaccharides. To identify the difference in biological activities of lipopolysaccharide of Pantoea agglomerans from other lipopolysaccharides on the basis of structure, we determined the structure of the lipid A part, which is the biological center of lipopolysaccharide, by quantitative analysis, nuclear magnetic resonance spectroscopy and mass spectrometry. Lipopolysaccharide of Pantoea agglomerans is constructed with at least two kinds of lipid A of different levels of acylation. One is of the same type as that of Escherichia coli with hexa‐acyl lipid A and the other is the Salmonella minnesota type with hepta‐acyl lipid A.