Open AccessRegulation of cyclodextrin glucanotransferase synthesis in Bacillus ohbensis
Author(s) -
Nishida Tamotsu,
Nakamura Akira,
Masaki Haruhiko,
Uozumi Takeshi
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10332.x
Subject(s) - starch , cyclodextrin , biochemistry , western blot , transcription (linguistics) , gene , chemistry , gene expression , blot , protein biosynthesis , northern blot , biology , philosophy , linguistics
The production of cyclodextrin glucanotransferase (CGTase) by Bacillus ohbensis is dependent on the presence of starch and inhibited by glucose in the medium. Northern blot analysis revealed that the CGTase gene ( cgt ) was transcribed to almost the same level irrespective of the presence or absence of starch, but glucose completely repressed the transcription. Furthermore, a relatively high amount of CGTase protein was detected on Western blotting only in the medium with starch, showing the lack of posttranslational control of the CGTase activity. These findings suggest some starch induction mechanism for the cgt gene, possibly at the posttranscriptional level, besides negative transcriptional regulation by glucose.