Open AccessCloning and transcriptional analysis of the lipA (lipoic acid synthetase) gene from Rhizobium etli
Author(s) -
Taté Rosarita,
Riccio Anna,
Iaccarino Maurizio,
Patriarca Eduardo J
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10324.x
Subject(s) - biology , lipoic acid , gene , mutant , transcription (linguistics) , microbiology and biotechnology , rnase p , promoter , homology (biology) , rhizobium , escherichia coli , genetics , biochemistry , gene expression , rna , linguistics , philosophy , antioxidant
We report here the isolation of a Rhizobium etli gene involved in lipoic acid metabolism, the lipA gene, which complements a lipA mutant strain of Escherichia coli . A promoter region ( lipA p) was mapped immediately upstream of lipA and two in vivo transcription initiation sites were identified, preceded by sequences showing some homology to the −10/−35 promoter consensus sequences. The activity of the lipA p was found not to be regulated either by the carbon source or by the addition of lipoic acid. Moreover, quantitative analysis of the lipA transcript by RNase protection assays indicated its down‐regulation during entry into stationary phase.