Open AccessProduction of multiple laccase isoforms by Phanerochaete chrysosporium grown under nutrient sufficiency
Author(s) -
Dittmer Joy K.,
Patel Nirav J.,
Dhawale Shrikrishna W.,
Dhawale Shree S.
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10309.x
Subject(s) - phanerochaete , laccase , chrysosporium , extracellular , size exclusion chromatography , chromatography , chemistry , intracellular , biochemistry , enzyme
Phanerochaete chrysosporium strain ME446 produced laccase when grown in a medium with high nitrogen (24 mM), glucose, acetate buffer and high copper (0.4 mM CuSO 4 ). Copper appeared to serve as an inducer. The highest amount of laccase activity was found in 2–3 day old cultures. Both intracellular and extracellular laccases eluted as four peaks (L1–L4) on a MonoQ (anion exchange) column. All four peaks from intracellular fluid appear to comprise multiple polypeptides that gave rise to a ∼400 kDa peak on a gel filtration column. Extracellular laccases had a non‐denaturing M r of 100 kDa.