Open AccessFabatins: new antimicrobial plant peptides
Author(s) -
Zhang Ying,
Lewis Kim
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10308.x
Subject(s) - candida albicans , antimicrobial , biochemistry , vicia faba , peptide , high performance liquid chromatography , amino acid , bacteria , chemistry , ion chromatography , antimicrobial peptides , chromatography , biology , microbiology and biotechnology , botany , genetics
Two new antimicrobial peptides related to the γ‐thionine family have been isolated by acid extraction from the broad bean Vicia faba . The extract was separated by ion exchange chromatography, and a fraction showing antibacterial activity was further purified by reverse‐phase HPLC. Material from a single HPLC peak was sequenced and revealed the presence of two peptides differing by one amino acid. The peptides were named fabatins. They are 47 amino acids long, have an overall positive charge and contain 8 cysteines that probably form 4 disulfide bridges characteristic of the γ‐thionins. Fabatins were active against both Gram‐negative and Gram‐positive bacteria, but were inactive against the yeasts Saccharomyces cerevisiae and Candida albicans .