Open AccessOctanoic acid uptake in Pseudomonas putida U
Author(s) -
Carnicero David,
FernándezValverde Martiniano,
Cañedo Librada M,
Schleissner Carmen,
Luengo José M
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10307.x
Subject(s) - pseudomonas putida , chemistry , fatty acid , intracellular , pseudomonas , phosphate , pseudomonadales , polymer , carbon fibers , biochemistry , nuclear chemistry , chromatography , organic chemistry , bacteria , biology , enzyme , materials science , genetics , composite number , composite material
Pseudomonas putida U grown in a chemically defined medium containing octanoic acid as the sole carbon source accumulated a homopolymer of poly(3‐hydroxyoctanoate) as intracellular reserve material, and metabolized the polymer during the late exponential phase of growth. Kinetic measurement of the uptake of [1‐ 14 C]octanoic acid by cells at 34°C in 85 mM phosphate buffer, pH 7.0 showed linear uptake for at least 2 min and the calculated K m and V max were 100 μM and 9 nmol min −1 respectively. This transport system is constitutive, energy‐dependent, and is strongly inhibited by structural analogs of octanoic acid, by various fatty acids with a carbon length higher than C 5 and by certain phenyl derivatives.