Open AccessListeria monocytogenes infection of HeLa cells results in listeriolysinO‐mediated transient activation of the Raf‐MEK‐MAP kinase pathway
Author(s) -
Weiglein Inge,
Goebel Werner,
Troppmair Jakob,
Rapp Ulf R,
Demuth Andreas,
Kuhn Michael
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10287.x
Subject(s) - listeria monocytogenes , kinase , mitogen activated protein kinase , mitogen activated protein kinase kinase , map kinase kinase kinase , cyclin dependent kinase 9 , map2k7 , mapk/erk pathway , mapk14 , mapkapk2 , ask1 , phosphorylation , cyclin dependent kinase 2 , microbiology and biotechnology , chemistry , biology , protein kinase a , genetics , bacteria
In this study we investigated the effect of Listeria monocytogenes infection on the activation of the Raf‐MEK‐MAP kinase pathway in eukaryotic host cells. HeLa cell infection with L. monocytogenes EGD resulted in a rapid, but transient, phosphorylation of the MAP kinases erk‐1 and erk‐2, a transient phosphorylation of the MAP kinase kinase MEK‐1, and a transient activation of the MAP kinase kinase kinase Raf. In parallel to the transient phosphorylation of the MAP kinases, we detected induced expression of the MAP kinase phosphatase MKP‐1. Additionally we present evidence that listeriolysinO is the inducing agent for activation of the Raf‐MEK‐MAP kinase pathway.