Open AccessTransformation of Thermoanaerobacterium sp. strain JW/SL‐YS485 with plasmid pIKM1 conferring kanamycin resistance
Author(s) -
Mai Volker,
Lorenz W.Walter,
Wiegel Juergen
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10283.x
Subject(s) - kanamycin , plasmid , transformation (genetics) , microbiology and biotechnology , strain (injury) , biology , genetics , gene , antibiotics , anatomy
The industrial application of thermophilic (eu)bacteria is hampered by the lack of genetic systems for these bacteria. We report here the first unequivocal transformation of a Gram‐positive, thermophilic, anaerobic microorganism, Thermoanaerobacterium , with the kanamycin resistance‐mediating plasmid pIKM1. The construct pIKM1 is based on the Escherichia coli–Clostridium acetobutylicum shuttle vector pIMP1 and contains the thermostable kanamycin cassette from S. faecalis plasmid pKD102. Using electrotransformation, plasmid pIKM1 mediated kanamycin resistance in Thermoanaerobacterium sp. strain JW/SL‐YS485 up to 400 μg ml −1 at 48°C and 200 μg ml −1 at 60°C.