Open AccessSequence diversity of a gene encoding a putative primary sigma factor among Borrelia burgdorferi sensu lato strains
Author(s) -
Pan Ming Jeng,
Tsai Ching Ping,
Yeh Jer Chih
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10281.x
Subject(s) - borrelia burgdorferi , biology , sensu , restriction fragment length polymorphism , genetics , sequence analysis , gene , nucleic acid sequence , polymerase chain reaction , spirochaetaceae , zoology , antibody , genus
Twenty‐six strains of Borrelia burgdorferi sensu lato were subjected to polymerase chain reaction (PCR)‐based restriction fragment length polymorphism (RFLP) analysis for assessing the sequence divergence of rpoD gene encoding the primary sigma factor. Four and five RFLP patterns were observed from two fragments of rpoD gene. Sequence analysis of a subgenic fragment covering region 1 through 4 from 13 strains of Borrelia burgdorferi s. l. revealed that 21 of 450 deduced amino acid residues were diverged. These results indicate that the sequence heterogeneity of rpoD is present in different strains of Borrelia burgdorferi s. l., and agreed well with the current classification of genospecies.