Open AccessA dyadic plasmid that shows MLS and PMS resistance in Staphylococcus aureus
Author(s) -
Matsuoka Mayumi,
Endou Kikutarou,
Kobayashi Hiroyuki,
Inoue Matsuhisa,
Nakajima Yoshinori
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10272.x
Subject(s) - msra , plasmid , staphylococcus aureus , microbiology and biotechnology , oleandomycin , erythromycin , antibiotics , biology , macrolide antibiotics , gene , strain (injury) , bacteria , genetics , amino acid , anatomy , methionine
Out of a collection of 56 Staphylococcus aureus clinical strains from 1971 to 1990 in Japan, we found one 1971 isolate, strain MS8968, harboring plasmid pMS97. A transductant strain, MS15009(pMS97), showed inducible resistance to a group of drugs, the so‐called MLS antibiotics in the presence of a low concentration of erythromycin (EM). However, in the case of oleandomycin (OL), the strain showed resistance to another group of antibiotics: 14‐membered macrolides (EM and OL), a 16‐membered macrolide (mycinamicin I), and type B streptogramin, the so‐called PMS antibiotics. Moreover, plasmid pMS97 contained an erm gene with universal primers specific for erm A , AM , B , BC , C , C ′, and G and an msrA gene with primers specific for msrA . The first finding suggests that two genes encoding functionally different mechanisms for MLS and PMS resistance, erm and msrA , are present together within plasmid pMS97 originating from S. aureus .