Open AccessEstablishing a model to study the regulation of the lactose operon in Lactobacillus casei
Author(s) -
Gosalbes María José,
Monedero Vicente,
Alpert CarlAlfred,
PérezMartínez Gaspar
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10271.x
Subject(s) - l arabinose operon , operon , gal operon , lactobacillus casei , lac operon , lactose , biology , trp operon , antitermination , plasmid , biochemistry , mutant , genetics , microbiology and biotechnology , bacteria , gene
The chromosomally encoded lactose‐specific phosphoenol pyruvate‐dependent phosphotransferase system (PTS) has been investigated in Lactobacillus casei ATCC 393 [pLZ15 ‐ ] and it was considered an excellent system to study the regulation of the lactose operon. This chromosomal operon has been cloned and sequenced, being 99% homologous to that encoded on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15 − ] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repression possibly mediated by CcpA and PTS elements, and (ii) induction by lactose through transcriptional antitermination.