Open AccessActinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia
Author(s) -
Tascón Cabrero RubénIván,
VázquezBoland José A.,
Gutiérrez César B.,
RodríguezBarbosa J.Ignacio,
RodríguezFerri Elías Fernando
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10266.x
Subject(s) - actinobacillus pleuropneumoniae , pleuropneumonia , microbiology and biotechnology , actinobacillus , chemistry , biology , bacteria , serotype , genetics
The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease‐negative mutant was isolated following transposon mutagenesis with a mini‐Tn 10 derivative. Both the parent strain and the urease‐negative mutant exhibited identical LD 50 values in a murine infection model. Pig challenge confirmed that the urease‐negative mutant was fully virulent, since experimental inoculation with 5×10 7 colony forming units resulted in an acute disease indistinguishable from that produced by the wild‐type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.