Open AccessCloning and sequencing of the gene encoding the AmpC β‐lactamase of Morganella morganii
Author(s) -
Barnaud Guilène,
Arlet Guillaume,
Danglot Claude,
Philippon Alain
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10260.x
Subject(s) - morganella morganii , cloning (programming) , microbiology and biotechnology , gene , biology , genetics , molecular cloning , computational biology , enterobacteriaceae , escherichia coli , peptide sequence , computer science , programming language
The chromosomal β‐lactamase gene of a clinical isolate of Morganella morganii was cloned in Escherichia coli and sequenced. The β‐lactamase had a pI of 7.4 and conferred a typical AmpC susceptibility pattern. The insert obtained was found to encode a protein of 379 amino acids. Its deduced amino acid sequence revealed it to be a class C β‐lactamase: 39–56% identity with chromosomal AmpC β‐lactamases of Serratia marcescens , Yersinia enterocolitica , Citrobacter freundii , Enterobacter cloacae and Escherichia coli ; and 37–56% identity with plasmid‐mediated β‐lactamases (MOX‐1, CMY‐1, FOX‐1, ACT‐1, LAT‐1, BIL‐1 and CMY‐2). The amp C gene was linked to a gene only part of which (450 bp) was cloned homologous to the regulatory ampR genes of chromosomal class C β‐lactamases.