Open AccessCloning and characterization of the recA gene of Paracoccus denitrificans and construction of a recA ‐deficient mutant 1
Author(s) -
Fernandez de Henestrosa Antonio R,
Rey Alfonso,
Tarragó Raül,
Barbé Jordi
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10243.x
Subject(s) - paracoccus denitrificans , rhodobacter , biology , gene , rhodobacter sphaeroides , nucleic acid sequence , plasmid , sequence analysis , genetics , microbiology and biotechnology , mutant , bacteria
The recA gene of Paracoccus denitrificans has been isolated from a genomic library by hybridization with the Rhodobacter sphaeroides recA gene. Its complete nucleotide sequence consists of 1071 bp encoding a polypeptide of 356 amino acids. Nucleotide sequence analysis of the P. denitrificans recA gene revealed the closest identities with the R. sphaeroides and the Rhodobacter capsulatus recA genes. Nevertheless, and surprisingly, recA genes of these two phototrophic bacteria are not DNA damage‐inducible when introduced into P. denitrificans cells, whereas recA genes of both P. denitrificans and Rhizobium etli are. These results suggest that the promoters of P. denitrificans and R. etli recA genes have a similar regulatory sequence. A recA ‐defective mutant of P. denitrificans has also been constructed by replacement of the active recA gene by an in vitro inactivated gene copy.