Open AccessIdentification and differentiation of mycobacteria using the P AN promoter sequence from Mycobacterium paratuberculosis as a DNA probe
Author(s) -
Gormley Eamonn,
Sandall Laurie,
Hong Cai,
Lawton David,
Murray Alan
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10221.x
Subject(s) - biology , mycobacterium , mycobacterium bovis , paratuberculosis , microbiology and biotechnology , restriction fragment length polymorphism , genomic dna , hybridization probe , genetics , mycobacterium tuberculosis , mycobacterium kansasii , dna , mycobacterium tuberculosis complex , dna sequencing , sequence analysis , gene , genotype , tuberculosis , bacteria , medicine , pathology
A 165 bp DNA fragment containing the P AN promoter from Mycobacterium paratuberculosis was used as a probe in Southern blots to detect the presence of related sequences in other species of mycobacteria. Among the species tested homologous sequences appeared to be present in representative pathogens belonging to the Mycobacterium tuberculosis complex, the MAIS complex, Mycobacterium kansasii and also the non‐pathogenic vaccine strain Mycobacterium bovis BCG. In addition, the probe could differentiate between these species on the basis of a restriction fragment length polymorphism (RFLP). No hybridization was observed with DNA extracted from a selected group of other slow‐growing and fast‐growing mycobacteria nor from a selection of other bacterial pathogens. It appears that the P AN sequence is identifying genomic regions common to the major pathogenic groups of mycobacteria.