Open AccessNon‐motile mini‐transposon mutants of Bordetella bronchiseptica exhibit altered abilities to invade and survive in eukaryotic cells
Author(s) -
West Nicholas P,
Fitter John T,
Jakubzik Ute,
Rohde Manfred,
Guzmán Carlos A,
Walker Mark J
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10203.x
Subject(s) - bordetella bronchiseptica , flagellin , mutant , flagellum , biology , transposon mutagenesis , motility , transposable element , mutagenesis , microbiology and biotechnology , bordetella , gene , genetics , bacteria , bordetella pertussis
Non‐motile mutants of Bordetella bronchiseptica were generated after mini‐transposon mutagenesis. One non‐motile mutant (designated VMM1) was derived from the bvg ‐positive strain BB7865 and four mutants (designated AMM1–4) were derived from the isogenic bvg ‐negative strain BB7866. Southern hybridisation analysis indicated that loss of motility was not due to the disruption of the flagellin subunit gene. Western blot and transmission electron microscopic analysis indicated that three of the five mutants expressed neither the flagellin subunit (40 kDa) nor flagella whereas one mutant expressed intact flagella under all conditions tested. One unique bvg ‐negative mutant, AMM4, exhibited temperature‐dependent repression of flagella biosynthesis and motility at 37°C. The ability of AMM4 to invade and survive in HeLa cells was significantly decreased.