Open AccessImmune response to vaccination with DNA encoding the bovine viral diarrhea virus major glycoprotein gp53 (E2)
Author(s) -
Harpin Serge,
Talbot Brian,
Mbikay Majambu,
Elazhary Youssef
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10198.x
Subject(s) - virology , virus , biology , dna vaccination , immune system , neutralizing antibody , recombinant dna , vaccination , antibody , microbiology and biotechnology , plasmid , pathogen , genotype , bovine herpesvirus 1 , glycoprotein , immunization , herpesviridae , dna , immunology , viral disease , gene , genetics , biochemistry
Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle which has not been controlled by classical vaccination. The region encoding the BVDV major glycoprotein gp53 (E2) known to possess virus‐neutralizing activity was cloned into a mammalian expression vector under the human cytomegalovirus (CMV) intermediate early promoter. Intramuscular and intradermal administration of the recombinant plasmid DNA into BALB/c mice induced BVDV gp53‐specific antibody responses to both biotypes (cytopathic and noncytopathic) of BVDV genotype 1, and to cytopathic BVDV genotype 2. BVDV‐neutralizing antibodies were generated against BVDV genotype 1 strains and they also persisted 6 months after the last injection.