Open AccessA novel plasmid‐encoded phage abortive infection system from Lactococcus lactis biovar. diacetylactis
Author(s) -
Deng YiMo,
Harvey Melissa L,
Liu ChunQiang,
Dunn Noel W
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10185.x
Subject(s) - plasmid , biovar , lactococcus lactis , biology , ribosomal binding site , open reading frame , gene , genetics , bacteriophage , microbiology and biotechnology , ribosome , bacteria , peptide sequence , rna , escherichia coli , lactic acid
A 16‐kb plasmid (pND859) was identified from Lactococcus lactis biovar. diacetylactis UK12922 which encodes phage resistance to the small isometric phage 712 when tested in L. lactis LM0230. The gene encoding phage abortive infection, designated abi‐ 859, was localized on a 1.2‐kb region which consists of an open reading frame (ORF) of 846 bp preceded by a potential ribosome‐binding site and a putative promoter region. A helix‐turn‐helix region typical of DNA‐binding motifs was identified near the N‐terminal of the abi‐ 859 product, suggesting a possible interaction with the phage DNA.