Open AccessA cluster of atypical Yersinia strains with a distinctive 16S rRNA signature
Author(s) -
Ibrahim Ashraf,
Liesack Werner,
Steigerwalt Arnold G,
Brenner Don J,
Stackebrandt Erko,
RobinsBrowne Roy M
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb10173.x
Subject(s) - 16s ribosomal rna , yersinia enterocolitica , biology , yersinia , typing , microbiology and biotechnology , sequence analysis , genetics , bacteria , gene
Thirty‐eight bacterial isolates from raw milk samples in Queensland, Australia were identified as members of the genus Yersinia on the basis of biochemical profile, ability to hybridize with a genus‐specific DNA probe, comparative 16S rDNA sequence analysis, and the presence of characteristic 16S rDNA signature nucleotides which occur in all Yersinia spp. Twenty‐five of these isolates reacted with typing sera (O:22 or O:58) of Y. enterocolitica ; the remainder were non‐typable. None of the isolates displayed any of the phenotypic or genetic virulence‐associated characteristics of Y. enterocolitica. Comparative 16S rDNA sequence analysis revealed that members of this group appear to represent a new sub‐line within the genus Yersinia , most closely related to Y. frederiksenii hybridization group 2 (unnamed genomospecies 2). This finding was confirmed by DNA hybridization studies which indicated that the strains belonged to the unnamed genomospecies, Yersinia frederiksenii genomospecies 2, which is biochemically indistinguishable from Y. frederiksenii ( Y. frederiksenii genomospecies 1). A 23‐nucleotide 16S rDNA signature stretch which characterised these strains was identified.