Open AccessPseudo‐transposition of a Tn5 derivative in Neisseria gonorrhoeae
Author(s) -
Thomas Christopher E.,
Carbonetti Nicholas H.,
Sparling P. Frederick
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08603.x
Subject(s) - transposase , neisseria gonorrhoeae , transposition (logic) , transposable element , insertion sequence , genetics , transformation (genetics) , biology , tn3 transposon , mutagenesis , p element , mutation , gene , mutant , computer science , artificial intelligence
We constructed a Tn5 derivative for potential use in transposon mutagenesis of Neisseria gonorrhoeae . It was incorporated into the chromosome apparently at random following transformation, but the insertion events were dependent on a functional RecA and independent of a functional transposase. Furthermore, in most cases there was an incomplete transposon inserted with little or no IS50 insertion sequence. These observations suggest that TnJ transposition may not be possible in N. gonorrhoeae and that this organism may have an unexplored illegitimate recombination system.