Open AccessProduction and purification of Clostridium perfringens alpha‐toxin using a protein‐hyperproducing strain, Bacillus brevis 47
Author(s) -
Nagahama Masahiro,
Michiue Kei,
Sakurai Jun
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08584.x
Subject(s) - clostridium perfringens , toxin , microbiology and biotechnology , biology , bacillus subtilis , signal peptide , gene , bacillaceae , bacillales , recombinant dna , bacteria , biochemistry , genetics
Clostridium perfringens alpha‐toxin was produced in a protein‐hyperproducing strain, Bacillus brevis 47, by cloning the gene into the constructed expression‐secretion vector which has the multiple promoters and the signal peptide coding region of an outer cell wall protein gene. The amount of alpha‐toxin produced by the B. brevis 47 transformant carrying the gene was approximately 10 times greater than that produced by a B. subtilis transformant carrying the toxin gene. Biological activities and the N‐terminal amino acid sequence of the toxin secreted by the B. brevis 47 transformant were identical to those of wild‐type alpha‐toxin.