Open AccessPromotion of Salmonella typhimurium adherence and membrane ruffling in MDCK epithelia by staurosporine
Author(s) -
Reed Katharine A.,
Booth Trevor A.,
Hirst Barry H.,
Jepson Mark A.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08583.x
Subject(s) - membrane ruffling , staurosporine , microbiology and biotechnology , salmonella , chemistry , biology , bacteria , signal transduction , biochemistry , protein kinase c , cell , genetics , cytoskeleton
Infection of Madin‐Darby canine kidney epithelial cell monolayers with Salmonella typhimurium SL1344 for 60 min results in widespread bacterial invasion which is associated with remodelling of the apical cell membrane to form ‘membrane ruffles’. Treatment of Madin‐Darby canine kidney cell monolayers with the protein kinase inhibitor staurosporine resulted in a 12‐fold increase in the number of adhered bacteria without significantly affecting bacterial invasion. Staurosporine treatment also significantly increased both the number and size of membrane ruffles. As S. typhimurium adhere preferentially to these areas of membrane lacking microvilli, the increased extent of membrane ruffling may explain the increased bacterial adherence. These data provide evidence that the propagation of membrane ruffles during S. typhimurium infection is modulated by changes in the phosphorylation state of host proteins.