Open AccessExpression in a wine yeast strain of the Aspergillus niger abfB gene
Author(s) -
Sáncheztorres Paloma,
Gonzálezcandelas Luis,
Ramón Daniel
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08576.x
Subject(s) - aspergillus niger , yeast , recombinant dna , heterologous expression , wine , heterologous , fermentation , strain (injury) , saccharomyces cerevisiae , biology , biochemistry , microbiology and biotechnology , gene , aspergillus , yeast in winemaking , chemistry , food science , anatomy
A recombinant wine yeast strain has been constructed expressing the gene coding for a‐L‐arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous α‐l‐arabinofuranosidase B shows similar physico‐chemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The a‐L‐arabinofuranosidase B protein is detected throughout the fermentation.