Open AccessOxygen increases the steady‐state level of nitrate in denitrifying cells of, Paracoccus denitrificans
Author(s) -
Kucera Igor,
Kaplan Petr,
Zeman Alois
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08572.x
Subject(s) - paracoccus denitrificans , denitrifying bacteria , nitrate , nitrite , denitrification , oxygen , chemistry , respiration , environmental chemistry , biochemistry , chromatography , botany , biology , nitrogen , organic chemistry , enzyme
The levels of nitrate in denitrifying cells of Paracoccus denitrificans were determined by centrifugation through silicone oil into phosphoric acid and ion‐exchange HPLC analysis of the cell lysates, using [ 14 C]sucrose to correct for the trapped external medium. Introduction of oxygen brought about a significant upward shift in the intracellular nitrate concentration. This result calls into question the current thinking that oxygen blocks nitrate respiration primarily due to the inhibition of nitrate transport into the cell.