Open AccessMolecular cloning and characterization of a Candida albicans gene ( EFB1 ) coding for the elongation factor EF‐1β
Author(s) -
Maneu Victoria,
Cervera Ana M.,
Martínez JoséP.,
Gozalbo Daniel
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08571.x
Subject(s) - biology , complementary dna , microbiology and biotechnology , elongation factor , cdna library , nucleic acid sequence , genetics , peptide sequence , genomic dna , gene , genomic library , intron , molecular cloning , sequence analysis , open reading frame , accession number (library science) , candida albicans , genbank , rna , ribosome
A Candida albicans gene homologous to Saccharomyces cerevisiae elongation factor 1β was isolated by screening a genomic DNA library using a C. albicans cDNA as a probe. This cDNA was previously obtained by immunoscreening of an expression library with polyclonal antibodies raised against candidal cell wall components. Sequence analysis of the cDNA and the whole C. albicans gene (EMBL accession number X96517) revealed an intron‐interrupted open reading frame of 639 base pairs that encodes a 213 amino acid protein. Exon sequences are highly homologous (74%) to S. cerevisiae EFB1 , whereas intron sequence is less conserved (34% identity), and the predicted amino acid sequence shares about 73% identity.