Open AccessConstruction of GFP vectors for use in Gram‐negative bacteria other than Escherichia coli
Author(s) -
Matthysse Ann G.,
Stretton Serina,
Dandie Catherine,
McClure Nicholas C.,
Goodman Amanda E.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08561.x
Subject(s) - escherichia coli , green fluorescent protein , plasmid , biology , bacteria , microbiology and biotechnology , enterobacteriaceae , gram negative bacteria , gene , genetics
A set of vectors containing a mutated gfp gene was constructed for use with Gram‐negative bacteria other than Escherichia coli . These constructs were: pTn 3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini‐Tn 5gfp for making random promoter probe gfp insertions directly into host strains; p519 gfp and p519 ngfp , broad host range mob + plasmids containing gfp from a lac and an npt 2 promoter, respectively.