Open AccessCloning and characterization of chitin synthase gene fragments from Penicillium chrysogenum
Author(s) -
Namgung Jun,
Park Bum Chan,
Lee Dong Hoon,
Bae Kyung Sook,
Park HeeMoon
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08558.x
Subject(s) - penicillium chrysogenum , chitin synthase , biology , genomic dna , gene , cloning (programming) , molecular cloning , genetics , chitin , microbiology and biotechnology , penicillium , sequence analysis , amino acid , biochemistry , peptide sequence , atp synthase , dna , chitosan , computer science , programming language
DNA fragments homologous to chitin synthase were amplified from the genomic DNA of Penicillium chrysogenum by PCR. Cloning and sequencing of the PCR‐amplified fragments led to the identification of four different genes, designated PcCHSI, PcCHS2, PcCHS3 , and PcCHS4 . By comparison of the deduced amino acid sequences, PcCHS1 was identified as a gene for class I chitin synthase, PcCHS2 and PcCHS3 were for class II, and PcCHS4 was for class III. Among these only PcCH4 includes an intervening sequence at 56 bp. The analysis of the deduced amino acid sequences revealed a close evolutionary relationship between Penicillium and ascomycetous fungi.