Open AccessRole of cytochrome b 562 in the archaeal aerobic respiratory chain of Sulfolobus sp. strain 7
Author(s) -
Iwasaki Toshio,
Oshima Tairo
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08539.x
Subject(s) - cytochrome , cyanide , biochemistry , respiratory chain , cytochrome c oxidase , electron transport chain , strain (injury) , sulfolobus , chemistry , biology , archaea , enzyme , inorganic chemistry , anatomy , gene
The role of cytochrome b 562 , a fragile constituent of the respiratory terminal oxidase supercomplex of the thermoacidophilic archaeon, Sulfolobus sp. strain 7, was investigated spectroscopically in the membrane‐bound state. Cytochrome b 562 did not react with CO or cyanide in the membrane‐bound state, while it was irreversibly modified to a CO‐reactive form ( b 562 ) upon solubilization in the presence of cholate and LiCl. Cyanide titration analyses with the succinate‐reduced membrane suggested that cytochrome b 562 was upstream of both the ‘ g y = 1.89’ Rieske FeS cluster and the a ‐type cytochromes. These results show that the b ‐type cytochrome functions as an intermediate electron transmitter in the terminal oxidase supercomplex.