Open AccessMetR‐mediated repression of the glyA gene in Escherichia coli
Author(s) -
Lorenz Eva,
Stauffer George V.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08535.x
Subject(s) - psychological repression , biology , mutant , transcription (linguistics) , gene , dna , escherichia coli , rna polymerase , genetics , gene expression , microbiology and biotechnology , philosophy , linguistics
Inactivation of either of the two MetR binding sites centered at bp —143 and —121 relative to the +1 transcription start site results in reduced glyA‐lacZ expression in a wild‐type strain below the level seen in a metR mutant. This reduced expression is dependent on the side of the DNA helix MetR binds relative to the RNA polymerase binding site. Thus, a single MetR dimer bound to the DNA may play a physiological role in maintaining appropriate glyA gene expression, functioning as a represser under low MetR conditions.