Open AccessIdentification and sequencing of a novel insertion sequence, IS1471, in Burkholderia cepacia strain 2a
Author(s) -
Xia Xiaosong,
Smith Anthony R.W.,
Brace Ian J.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08531.x
Subject(s) - genetics , insertion sequence , biology , sequence (biology) , nucleic acid sequence , plasmid , inverted repeat , direct repeat , computational biology , dna sequencing , open reading frame , dna , sequence logo , peptide sequence , sequence analysis , microbiology and biotechnology , consensus sequence , base sequence , gene , transposable element , genome
A novel insertion sequence (IS), IS1471, has been identified which is inserted into IS element IS1071 possessed by plasmid pIJB1 in Burkholderia cepacia strain 2a. Nucleotide sequencing has revealed that IS1471 is 1112 bp in length and is flanked by 22/21‐bp imperfect inverted repeats with a 3‐bp duplication of the target sequence. IS1477 contains a single open reading frame encoding a putative polypeptide of 345 amino acids with molecular mass of 39 406 Da. Searches of DNA and protein sequence databases did not result in the detection of any homologous IS elements, suggesting that IS1471 is novel and may not belong to any known IS groups.