Open AccessMapping of noninvasion Tn phoA mutations on the Escherichia coli O18:K1:H7 chromosome
Author(s) -
Bloch Craig A.,
Huang ShengHe,
Rode Christopher K.,
Kim Kwang Sik
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08526.x
Subject(s) - escherichia coli , virulence , biology , transposable element , enterobacteriaceae , mutant , serotype , microbiology and biotechnology , gene , genetics , virology
The most virulent newborn meningitis‐associated Escherichia coli are of the serotype O18: K1: H7. We previously isolated a large number of E. coli O18:K1:H7 mutants resulting from transposon Tn phoA mutagenesis that fail to invade brain microvascular endothelial cells. We have now determined the locations of 45 independent insertions. Twelve were localized to the 98 min region, containing a 120 kb segment that is characteristic of E. coli O18:K1:H7. Another, the previously described insertion ibe ‐10::Tn phoA , was localized to the 87 min region, containing a 20 kb segment found in this E. coli . These noninvasion mutations may define new O18:K1:H7 pathogenicity islands carrying genes for penetration of the blood‐brain barrier of newborn mammals.