Open AccessComparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS‐PAGE and immunoblotting
Author(s) -
Tola Sebastiana,
Idini Graziano,
Manunta Daniela,
Casciano Ida,
Rocchigiani Angela M.,
Angioi Antonio,
Leori Guido
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08490.x
Subject(s) - xhoi , biology , gel electrophoresis , restriction enzyme , microbiology and biotechnology , antiserum , polyacrylamide gel electrophoresis , southern blot , antigen , hindiii , mycoplasma , genomic dna , mollicutes , dna , genetics , ecori , enzyme , biochemistry
Abstract We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS‐PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates was investigated analyzing hydrophobic membrane protein fractions by immunoblotting using pooled sheep antiserum from naturally infected sheep. Large restriction fragments obtained cleaving genomic DNAs with Sma I, Nru I, Sal I, Xho I, Bss HII and Kpn I were analyzed by pulsed field gel electrophoresis. Genetic analysis indicates that isolates are all similar without intraspecific differences. This homogeneity was confirmed by immunoblotting: 80 and 50 kDa antigens are present in all strains analyzed.