Open AccessInhibition of acid production in Streptococcus mutans R9 by formic acid
Author(s) -
Assinder S.J.,
Popiel H.A.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08485.x
Subject(s) - streptococcus mutans , formic acid , chemistry , acetic acid , formate , lactic acid , bacteria , biochemistry , in vivo , microbiology and biotechnology , biology , genetics , catalysis
Both lactic and acetic acids cause mixed inhibition of acid production in mutans streptococci. This inhibition is partly irreversible due to cell death, an important factor when considering acidogenicity and aciduricity of these organisms, and their role in the caries process. Other monocarboxylic end‐products may be present and may also be important inhibitors of acid production in dental plaque. This study considered the effects of varying concentrations of the end‐product formic acid on acid production rates in Streptococcus mutans R9, measured using the pH‐stat. Undissociated formic acid caused mixed inhibition with constants of K iu (uncompetitive) of 6.07 ± 1.27 mmol 1 −1 and K ic (competitive) of 0.2 ± 10.11 mmol I −1 . Inhibition was found to be fully reversible, with no loss of cell viability. It is concluded that at those concentrations found in vivo, formate is not a significant inhibitor of acid production by S. mutans in dental plaque at any time, and is not important in determining the acidogenicity or aciduricity of this organism.