Open AccessDetection of the response regulator AgrA in the cytosolic fraction of Staphylococcus aureus by monoclonal antibodies
Author(s) -
Morfeldt Eva,
PanovaSapundjieva Iscra,
Gustafsson Björn,
Arvidson Staffan
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08480.x
Subject(s) - agra , staphylococcus aureus , quorum sensing , virulence , biology , response regulator , gene , transcription (linguistics) , microbiology and biotechnology , monoclonal antibody , regulator , transcription factor , antibody , bacteria , genetics , bacterial protein , ecology , linguistics , philosophy
The expression of many virulence genes in Staphylococcus aureus is controlled by a regulatory RNA molecule, RNAIII, which is encoded by the agr locus. Transcription of RNAIII requires the activity of the agrA, B, C and D genes, which code for components of a quorum sensing signal transduction system. In this report we describe the overexpression and purification of the response regulator, AgrA. Monoclonal antibodies were produced and used to detect AgrA in the cytosolic fraction of S. aureus cells. Purified AgrA did not bind to the RNAIII promoter region in a DNA mobility shift experiment. This confirms previous results obtained with protein extracts from agr + and agr − cells.