Open AccessComparative DNA analysis of Bordetella pertussis clinical isolates by pulsed‐field gel electrophoresis, randomly amplified polymorphism DNA, and ERIC polymerase chain reaction
Author(s) -
Moissenet D.,
Valcin M.,
Marchand V.,
Grimprel E.,
Bégué P.,
GarbargChe A.,
VuThien H.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08471.x
Subject(s) - rapd , pulsed field gel electrophoresis , biology , polymerase chain reaction , bordetella pertussis , dna profiling , intergenic region , gel electrophoresis , microbiology and biotechnology , dna sequencer , dna , genetics , genotype , bacteria , genetic diversity , gene , genome , medicine , population , environmental health
We used DNA fingerprinting by pulsed‐field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) and PCR amplification of enterobacterial repetitive intergenic consensus sequences (ERIC‐PCR) to compare 15 clinical isolates of Bordetella pertussis recovered between August 1993 and September 1995 from 13 infants and two adults, living in the same geographic area. PFGE produced 10 patterns and made it possible to differentiate all the isolates and to indicate an intrafamilial transmission. RAPD and ERIC‐PCR generated banding patterns with small differences and had a poor discriminatory power. During the last 2 years, at Armand‐Troussau pediatric hospital, 10 distinct clones of clinical B. pertussis isolates, with a predominant clone including seven strains, could be determined by the PFGE method. skw] Bordetella pertussis ; Pulsed‐field gel electrophoresis; Randomly amplified polymorphic DNA; PCR amplification of enterobacterial repetitive intergenic consensus sequences